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Optimization of an Extraction Method for the Simultaneous Quantification of Sixteen Polyphenols in Thirty-One Pulse Samples by Using HPLC-MS/MS dynamic-MRM Triple Quadrupole

Optimization of an Extraction Method for the Simultaneous Quantification of Sixteen Polyphenols in Thirty-One Pulse Samples by Using HPLC-MS/MS dynamic-MRM Triple Quadrupole

Giovanni Caprioli, Franks Kamgang Nzekoue, Federica Giusti, Sauro Vittori, Gianni Sagratini

Food Chem. 2018 Nov 15;266:490-497.

PMID: 30381217

Abstract:

Pulses are an important source of proteins, carbohydrates, and dietary fibre, and also contain polyphenols, which are bioactive compounds with antioxidant activity. A new analytical method that uses high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed for the quantification of sixteen polyphenols in thirty-one pulse varieties. Different extraction procedures were tested (acidic hydrolysis, alkaline hydrolysis and extraction without hydrolysis), and acidic hydrolysis at pH 2, extraction temperature of 20 °C and extraction time of 2 h was proven to be the best in terms of recovery percentages (99.7-107.6%). The highest polyphenol levels were found in beans, particularly black beans (459 mg kg-1) and ruviotto beans (189 mg kg-1); significant levels of polyphenols were also observed in lentils, particularly black lentils (137 mg kg-1) and quality gold lentils (132 mg kg-1). This study provides new information about legume polyphenols, offering reasons to promote legumes as part of a healthy diet.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP17670063	Delphinidin 3,5-diglucoside chloride		17670-06-3	Price
AP7084244-A	Cyanidin 3-glucoside chloride		7084-24-4	Price

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