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Optimization of Growth Conditions for the Isolation of Dextran Producing Leuconostoc Spp. From Indigenous Food Sources

Optimization of Growth Conditions for the Isolation of Dextran Producing Leuconostoc Spp. From Indigenous Food Sources

Farwa Sarwat, Nuzhat Ahmed, Afsheen Aman, Shah Ali Ul Qader

Pak J Pharm Sci. 2013 Jul;26(4):793-7.

PMID: 23811460

Abstract:

Leuconostoc are known to produce dextran, which have great commercial importance in chemical, medical and food industry. The present study is an attempt to select the best medium for the isolation of indigenous dextran producing Leuconostoc, measuring their enzyme activities for dextransucrase, production of dextran and identification of dextran producing Leuconostoc CMG706, CMG707, CMG710 and CMG713. Since, dextran producing Leuconostoc produce slimy colonies, twenty-four slime producing bacterial strains were isolated from different food sources, fruits and vegetables. Three different isolation medium were evaluated for the isolation of Leuconostoc only and the best one was found to be one containing sucrose and sodium azide. Further, all slime producing bacterial strains were screened for enzyme activity of dextransucrase, which is responsible for dextran production. Four bacterial strains CMG706, CMG707, CMG710 and CMG713 giving high enzyme activities were selected for dextran production and identified.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9004540-C	Dextran from Leuconostoc spp.		9004-54-0	Price

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