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Organosoluble Enzyme Conjugates With poly(2-oxazoline)s via Pyromellitic Acid Dianhydride

Organosoluble Enzyme Conjugates With poly(2-oxazoline)s via Pyromellitic Acid Dianhydride

Stefan Konieczny, Christoph P Fik, Nils J H Averesch, Joerg C Tiller

J Biotechnol. 2012 Jun 15;159(3):195-203.

PMID: 22306109

Abstract:

The use of enzymes in organic solvents offers a great opportunity for the synthesis of complex organic compounds and is therefore in focus of current research. In this work we describe the synthesis of poly(2-methyl-1,3-oxazoline) (PMOx) and poly(2-ethyl-1,3-oxazoline) (PEtOx) enzyme conjugates with hen-egg white lysozyme, RNase A and α-chymotrypsin using a new coupling technique. The POXylation was carried out reacting pyromellitic acid dianhydride subsequently with ethylenediamine terminated POx and then with the NH₂-groups of the respective enzymes. Upon conjugation with the polymers, RNase A and lysozyme became fully soluble in DMF (1.4 mg/ml). These are the first examples of fully POXylated proteins, which become organosoluble. The synthesized enzyme conjugates were characterized by SDS-PAGE, isoelectric focusing, dynamic light scattering and size exclusion chromatography, which all indicated the full POXylation of the enzymes. The modified enzymes even partly retained their activity in water. With α-chymotrypsin as example we could demonstrate that the molecular weight of the attached polymer significantly influences the activity.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS775168	Poly(pyromellitic dianhydride-co-thionin)			Price

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