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Over-expression of Chloroperoxidase in Caldariomyces Fumago

Markus Buchhaupt, Kristina Ehrich, Sonja Hüttmann, Jan Guder, Jens Schrader

Biotechnol Lett. 2011 Nov;33(11):2225-31.

PMID: 21735258

Abstract:

The filamentous fungus Caldariomyces fumago secretes a chloroperoxidase (CPO). To increase its production, we integrated a CPO-expression cassette into the non-transcribed spacer regions of the rDNA in C. fumago. One strain was obtained that had twice the CPO activity when grown in shake-flask and bioreactor compared to the wild-type. The highest CPO activity from the bioreactor cultivation was 3,236 U ml(-1). This is the highest value reported so far.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP9055203 Chloroperoxidase from Caldariomyces fumago Chloroperoxidase from Caldariomyces fumago 9055-20-3 Price
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