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Paraoxon, 4-nitrophenyl Phosphate and Acetate Are Substrates of α- But Not of β-, γ- And ζ-carbonic Anhydrases

Paraoxon, 4-nitrophenyl Phosphate and Acetate Are Substrates of α- But Not of β-, γ- And ζ-carbonic Anhydrases

Alessio Innocenti, Claudiu T Supuran

Bioorg Med Chem Lett. 2010 Nov 1;20(21):6208-12.

PMID: 20833546

Abstract:

Carbonic anhydrases (CAs, EC 4.2.1.1) belonging to α-, β-, γ- and ζ-classes and from various organisms, ranging from the bacteria, archaea to eukarya domains, were investigated for their esterase/phosphatase activity with 4-nitrophenyl acetate, 4-nitrophenyl phosphate and paraoxon as substrates. Only α-CAs showed esterase/phosphatase activity, whereas enzymes belonging to the β-, γ- and ζ-classes were completely devoid of such activity. Paraoxon, the metabolite of the organophosphorus insecticide parathione, was a much better substrate for several human/murine α-CA isoforms (CA I, II and XIII), with k(cat)/K(M) in the range of 2681.6-4474.9M(-1)s(-1), compared to 4-nitrophenyl phosphate (k(cat)/K(M) of 14.9-1374.4M(-1)s(-1)).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP830035	4-Nitrophenyl acetate		830-03-5	Price

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