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Partial Purification and Characterization of Porcine Platelet-Derived Growth Factor (PDGF)

Partial Purification and Characterization of Porcine Platelet-Derived Growth Factor (PDGF)

A Poggi, B Rucinski, P James, J C Holt, S Niewiarowski

Exp Cell Res. 1984 Feb;150(2):436-41.

PMID: 6692858

Abstract:

Platelet derived growth factor (PDGF) has been partially purified from porcine platelets. Purification steps included heparin-agarose chromatography of the material released by thrombin-stimulated washed porcine platelets and Blue-Sepharose chromatography. Preparative isoelectric focusing showed that isoelectric point of porcine PDGF is at pH 10.0-11.0 and elution experiments from sodium dodecyl sulfate (SDS) polyacrylamide gels indicated that its molecular weight is close to 30 kD. The immunoglobulin fraction prepared from anti-human PDGF serum inhibited the mitogenic activity of porcine PDGF. These experiments suggest a homology of porcine and human PDGF. Porcine platelet factor 4 and porcine platelet basic protein were devoid of significant mitogenic activity.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248635	Platelet-Derived Growth Factor from porcine platelets			Price

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