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Patch Testing With Serial Dilutions and Thin-Layer Chromatograms of Oak Moss Absolutes Containing High and Low Levels of Atranol and Chloroatranol

Patch Testing With Serial Dilutions and Thin-Layer Chromatograms of Oak Moss Absolutes Containing High and Low Levels of Atranol and Chloroatranol

Martin Mowitz, Erik Zimerson, Cecilia Svedman, Magnus Bruze

Contact Dermatitis. 2013 Dec;69(6):342-9.

PMID: 24102141

Abstract:

Background:
Oak moss absolute (Evernia prunastri extract) contains a large number of substances, among them the potent allergens atranol and chloroatranol. Since 2008, their content in oak moss absolute has been restricted by the International Fragrance Association to a maximum level of 100 ppm each.
Objectives:
To compare the elicitation capacities of a traditional (sample A) and a treated (sample B) oak moss absolute containing, in total, 27 000 and 66 ppm of atranol and chloroatranol, respectively, and to investigate reactions to components of oak moss absolute separated by thin-layer chromatography (TLC).
Methods:
Fifteen oak moss-allergic subjects were patch tested with serial dilutions and TLC strips of samples A and B.
Results:
Fifteen subjects reacted to sample A at concentrations ≤ 2.0%, and 2 subjects reacted to sample B at 2.0% but not to lower concentrations. Among 13 subjects reacting to the TLC strip of sample A, 11 reacted to spots with retardation factor values corresponding to those of atranol and/or chloroatranol, and 11 reacted to other areas on the TLC strip. Only one subject reacted to the TLC strip of sample B.
Conclusions:
The patch test reactivity of sample B was significantly lower than that of sample A. The TLC patch tests indicate the presence of sensitizers other than atranol and chloroatranol in oak moss absolute.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP526374	Atranol		526-37-4	Price
AP90028685	Oakmoss Extract solution		90028-68-5	Price

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