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Pattern Recognition of 8-hydroxy-2'-deoxyguanosine in Biological Fluids

Pattern Recognition of 8-hydroxy-2'-deoxyguanosine in Biological Fluids

Raluca-Ioana Stefan-van Staden, Liliana-Roxana Balahura, Livia Alexandra Gugoasa, Jacobus F van Staden, Hassan Y Aboul-Enein, Marcela-Corina Rosu, Stela Maria Pruneanu

Anal Bioanal Chem. 2018 Jan;410(1):115-121.

PMID: 29067480

Abstract:

8-Hydroxy-2'-deoxyguanosine (8-OHdG), a product of oxidative DNA damage, which has been used as a sensitive and reliable marker of oxidative stress and carcinogenesis, is found in high levels in biological fluids of leukemia patients. A reliable screening method based on pattern recognition of 8-OHdG using stochastic sensors designed with graphene materials decorated with nanoparticles of TiO2Ag or TiO2Au was developed. 5,10,15,20-Tetraphenyl-21H,23H porphyrin (P), 2,6-bis((E)-2-(furan-2-yl)vinyl)-4-(4,6,8-trimethylazulen-1-yl)pyridine (Py1), and 2,6-bis((E)-2-(thiophen-3-yl)vinyl)-4-(4,6,8-trimethylazulen-1-yl)pyridine (Py2) were used as modifiers of the graphene paste in the design of sensors. The screening method used for pattern recognition was developed for two pH values accordingly with the nature of the biological fluid to be screened: pH = 3.02 for urine samples and pH = 7.49 for whole blood samples. High sensitivities and low limits of determination for 8-OHdG obtained at both pH values favored the early detection of leukemia. Recoveries over 98.00% with RSD (%) values lower than 1.00% proved the reliability of the proposed screening method. Graphical abstract Graphene based sensors detect 8-hydroxy-2'-deoxyguanosine in biological fluids.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP88847896	8-Hydroxy-2′-deoxyguanosine		88847-89-6	Price

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