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Peptide Substrate for caspase-3 With 2-aminoacridone as Reporting Group

Valentin Lozanov, Ivaylo P Ivanov, Bistra Benkova, Vanio Mitev

Amino Acids. 2009 Mar;36(3):581-6.

PMID: 18597040

Abstract:

Synthesis and properties of a new fluorescent/fluorogenic substrate Ac-DEVD-AMAC for caspase-3 are reported. The substrate is obtained by conventional Fmoc-based solid phase peptide synthesis and its properties are investigated with regard to fluorescence, sensitivity, applicability and kinetic constants. A non-traditional approach to assay the proteases activity using 2-aminoacridone labeled peptides is proposed. This approach utilizes the decrease of fluorescence intensity of a sample as a measure for the enzyme activity.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP27918145 2-Aminoacridone 2-Aminoacridone 27918-14-5 Price
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