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Peroxiredoxin V Reduces β-Lapachone-induced Apoptosis of Colon Cancer Cells

Peroxiredoxin V Reduces β-Lapachone-induced Apoptosis of Colon Cancer Cells

Yue Liu, Taeho Kwon, Ji-Su Kim, Nisansala Chandimali, Ying-Hua Jin, Yi-Xi Gong, Dan-Ping Xie, Ying-Hao Han, Mei-Hua Jin, Gui-Nan Shen, Dong Kee Jeong, Dong-Sun Lee, Yu-Dong Cui, Hu-Nan Sun

Anticancer Res. 2019 Jul;39(7):3677-3686.

PMID: 31262894

Abstract:

Background/aim:
Peroxiredoxin (Prx) V has been known as an antioxidant enzyme which scavenges intracellular reactive oxygen species (ROS). Also, Prx V has been shown to mediate cell apoptosis in various cancers. However, the mechanism of Prx V-induced apoptosis in colon cancer cells remains unknown. Thus, in this study we analyzed the effects of Prx V in β-lapachone-induced apoptosis in SW480 human colon cancer cells.
Materials and methods:
β-lapachone-induced apoptosis was analyzed by the MTT assay, western blotting, fluorescence microscopy, Annexin V staining and flow cytometry.
Results:
Overexpression of Prx V, significantly decreased β-lapachone-induced cellular apoptosis and Prx V silencing increased β-lapachone-induced cellular apoptosis via modulating ROS scavenging activity compared to mock SW480 cells. In addition, to further explore the mechanism of Prx V regulated β-lapachone-induced SW480 cells apoptosis, the Wnt/β-catenin signaling was studied. The Wnt/ β-catenin signaling pathway was found to be induced by β-lapachone.
Conclusion:
Prx V regulates SW480 cell apoptosis via scavenging ROS cellular levels and mediating the Wnt/β-catenin signaling pathway, which was induced by β-lapachone.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP4707328	β-Lapachone		4707-32-8	Price

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