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[Persistence of Type 1 Human Immunodeficiency Virus (HIV-1) in MT-4 Cells in Presence of Alpha-Interferon]

[Persistence of Type 1 Human Immunodeficiency Virus (HIV-1) in MT-4 Cells in Presence of Alpha-Interferon]

L M Selimova, S N Iordanskiĭ, E V Kazennova, A V Bobkov

Vopr Virusol. Mar-Apr 2001;46(2):17-20.

PMID: 11392964

Abstract:

The development of HIV infection during serial passages of HIV-1 strains with different replication capacity was studied in the presence of natural human alpha-interferon (IFN-alpha). The virus activity was evaluated by accumulation of virus-specific proteins in cells and culture fluid, determined by Western blot method and analysis of viral DNA in the cells by polymerase chain reaction. IFN-alpha suppressed replication of HIV-1/IIIB and 1974. The replication of strain 1974 (with a 10-fold lower replicative activity than strain IIIB) was inhibited during the first passage and of strain IIIb during the second passage. After the fourth passage IFN-2 alpha completely suppressed the replication of strain 1974, and the virus activity did not manifest after 4 consecutive co-culturings of cells from fourth viral passage with intact cells without IFN-alpha. A population of viral particles whose replicative activity was virtually undetected in the presence of IFN-alpha seemed to persist during serial passages of strain IIIB in MT-4 cells. On the other hand, the virus replication was restored during the first co-culturing of cells from the virus subculture with intact cells in the absence of IFN-alpha. Presumably, an increase in the level of IFN-alpha in the blood of HIV-infected patients at late stages of AIDS is not accidental and is caused by appearance of more virulent variants of the virus.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248762	IFN-alpha 1 human			Price

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