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Phenotype and Function of GM-CSF Independent Dendritic Cells Generated by Long-Term Propagation of Rat Bone Marrow Cells

Phenotype and Function of GM-CSF Independent Dendritic Cells Generated by Long-Term Propagation of Rat Bone Marrow Cells

Hua Yan, Tohko Miyagi, Eigo Satoh, Wataru Sugiura, Naoki Yamamoto, Hiromitsu Kimura

Cell Immunol. 2004 Jun;229(2):117-29.

PMID: 15474526

Abstract:

GM-CSF is believed to be an essential factor for growth and differentiation of myeloid dendritic cells (DC). Employing a low-density fraction of rat bone marrow cells, we attempted to generate DC with human Flt-3/Flk-2 and IL-6. In this culture system, typical DC gradually appeared without exogenous GM-CSF supplement. Phenotypes and functions of the DC were examined. Evidence provided that the most efficient long-term outgrowth of DC progenitors was obtained by GM-CSF independent culture systems with the aid of Flt3/Flk-2 and IL-6, not with c-kit ligand and IL-6. Furthermore, CD103 (OX-62), which is widely used for rat DC separation, was found to be insufficient for enriching DC, due to the down-regulation of the marker. However, the most efficient selection of rat DC was made by CD161a (NKR-P1A), a C-type lectin family. The GM-CSF independent DC was functionally active in vitro as well as in vivo assays.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248585	Flt-3/Flk-2 Ligand from mouse			Price

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