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Phenylboronic acid functionalized C 3 N 4 facultative hydrophilic materials for enhanced enrichment of glycopeptides

Phenylboronic acid functionalized C 3 N 4 facultative hydrophilic materials for enhanced enrichment of glycopeptides

Yong Zhang, Hongyu Jing, Tao Wen, Yao Wang, Yang Zhao, Xiangke Wang, Xiaohong Qian, Wantao Ying

Talanta. 2019 Jan 1;191:509-518.

PMID: 30262092

Abstract:

It is challenging to capture N-glycopeptides with high recovery and high specificity from complicated biosystems. Herein, we present a facile and economical procedure to generate a novel self-assembling 4-Mercaptobenzene boronic acid functionalized and Au-doped Straticulate C3N4 (MASC), with enhanced affinity capability towards glycopeptides. The materials possess low pH value adaptation, high hydrophilicity and stability, good repeatability and recyclability, and provided high selectivity (1:100), low limit of detection (0.33 fmol/μL), high enrichment efficiency (~ 80%) and high recovery rate (~ 90%) towards glycopeptides. The materials can capture glycopeptides unbiasedly, as demonstrated by the identification of 37 glycopeptides from IgG and 21 glycopeptides from horseradish peroxidase (HRP). The performance of MASC on human urine and serum glycoproteome analysis was also tested. An average of 1465 glycopeptides from 839 glycoproteins and 1553 glycopeptides from 884 glycoproteins were identified from female and male urine samples in a single mass spectrometry analysis. O-glycopeptides from human urine were also significantly enriched. Additionally, 463 glycopeptides assigned to 209 glycoproteins were identified from 5 μL of human serum. All of these results indicate that MASC presents a good performance and applicability in the field of glycoproteomic research.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS793961	3-(trans-4-Hydroxycyclohexylcarbamoyl)phenylboronic acid			Price
LS793974	4-(trans-4-Hydroxycyclohexylcarbamoyl)phenylboronic acid			Price

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