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Phenylboronic Acid-Functionalized Polymeric Micelles With a HepG2 Cell Targetability

Xiaojin Zhang, Zhenguo Zhang, Xin Su, Mengmeng Cai, Renxi Zhuo, Zhenlin Zhong

Biomaterials. 2013 Dec;34(38):10296-304.

PMID: 24075483

Abstract:

Phenylboronic acid-functionalized amphiphilic block copolymer Pluronic-PMCC-BA was synthesized via ring-opening polymerization of 5-methyl-5-benzyloxycarbonyl-1,3-dioxan-2-one (MBC) with fumaric acid as a catalyst followed by the deprotection of carboxyl groups by catalyzed hydrogenation and the condensation of 3-aminophenylboronic acid with the copolymer side groups. Pluronic-PMCC-BA can form stable micelle solution by self-assembly in water. The phenylboronic acid groups are located at the shell of micelle as proved by (1)H NMR. The diameter of drug-free micelles is approximate 60 nm. Nano-spheres with narrow size distribution could be observed in the TEM image. MTT assay results show that Pluronic-PMCC-BA exhibits slight cytotoxicity when the polymer concentration is higher than 25 μg mL(-1). The toxicities of DOX@Pluronic-PMCC and DOX@Pluronic-PMCC-BA to COS7, HeLa, and HepG2 cell lines are similar with those of free DOX. Interestingly, phenylboronic acid groups located at the surface of Pluronic-PMCC-BA micelles can recognize HepG2 cells and promote the drug uptake of the cells, which are observed by confocal laser scanning microscopy (CLSM). The results imply that Pluronic-PMCC-BA would be a promising material for targeted drug delivery to the cancer cells.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP1072951666 3-[(4′-tert-Butyl-2′-methylphenoxy)methyl]phenylboronic acid 3-[(4′-tert-Butyl-2′-methylphenoxy)methyl]phenylboronic acid 1072951-66-6 Price
AP1072951768 2-[(4′-tert-Butyl-2′-methylphenoxy)methyl]phenylboronic acid 2-[(4′-tert-Butyl-2′-methylphenoxy)methyl]phenylboronic acid 1072951-76-8 Price
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