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Phorbol 12-myristate 13-acetate (PMA) suppresses high Ca 2+-enhanced adipogenesis in bone marrow stromal cells

Phorbol 12-myristate 13-acetate (PMA) suppresses high Ca 2+-enhanced adipogenesis in bone marrow stromal cells

Ryota Hashimoto, Yuki Miyamoto, Seigo Itoh, Hiroyuki Daida, Takao Okada, Youichi Katoh

J Physiol Sci. 2019 Sep;69(5):741-748.

PMID: 31256364

Abstract:

We have previously reported that increased extracellular and intracellular Ca2+ lead to adipocyte accumulation in bone marrow stromal cells (BMSCs). However, strategies to suppress high Ca2+-enhanced adipocyte accumulation have not been reported. We examined the effects of the diacylglycerol analog phorbol 12-myristate 13-acetate (PMA) on proliferation and adipogenesis of mouse primary BMSCs. We used 9 mM CaCl2 and 100 nM ionomycin to increase extracellular Ca2+ and intracellular Ca2+, respectively. PMA suppressed the expression of both C/EBPα and PPARγ under normal adipogenesis, adipogenesis + CaCl2, and adipogenesis + ionomycin conditions. PMA enhanced proliferation under normal adipogenesis conditions but suppressed proliferation under adipogenesis + CaCl2 and adipogenesis + ionomycin conditions. PMA did not affect the accumulation of adipocytes under normal adipogenesis conditions but suppressed adipocyte accumulation under adipogenesis + CaCl2 and adipogenesis + ionomycin conditions. These results suggest that the PMA-dependent pathway is an important signaling pathway to suppress high Ca2+-enhanced adipocyte accumulation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP16561298-A	Phorbol 12-myristate 13-acetate		16561-29-8	Price

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