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Phospholipid-lactose Permease Interaction as Reported by a Head-Labeled Pyrene Phosphatidylethanolamine: A FRET Study

Phospholipid-lactose Permease Interaction as Reported by a Head-Labeled Pyrene Phosphatidylethanolamine: A FRET Study

Carme Suárez-Germà, Luís M S Loura, Manuel Prieto, Òscar Domènech, Josep M Campanera, M Teresa Montero, Jordi Hernández-Borrell

J Phys Chem B. 2013 Jun 6;117(22):6741-8.

PMID: 23647499

Abstract:

Förster resonance energy transfer (FRET) measurements were performed in preceding works to study the selectivity between a single-tryptophan mutant of lactose permease (LacY) of Escherichia coli (used as the donor) and phospholipid probes labeled with pyrene at the acyl chain moiety (used as the acceptor). In the present work, we report the results obtained by using the same LacY mutant (W151/C154G) and binary lipid mixtures of phosphatidylethanolamine (PE) differing in the acyl chain composition and 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (POPG) (3:1 mol/mol) doped with a phospholipid probe labeled with pyrene at the headgroup. The use of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(1-pyrenesulfonyl) ammonium salt (HPyr-PE), which bears two unsaturated acyl chains, enabled the investigation of the specific interaction between LacY and HPyr-PE. The main conclusions raised from our results suggest that (i) for phase-separated systems, LacY would be located in fluid domains nominally enriched in POPG, and if a given proportion of PE is present in this phase, it will be mainly located around LacY; and (ii) in the absence of phase separation, LacY is preferentially surrounded by PE and, in particular, seems to be sensitive to the lipid spontaneous curvature.

Chemicals Related in the Paper:

Catalog Number	Product Name	CAS Number	Price
AP108347804	1,2-Dioctadecanoyl-sn-glycero-3-phospho-rac-(1-glycerol) ammonium salt	108347-80-4	Price
AP267228706	2-Oleoyl-1-palmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) ammonium salt	267228-70-6	Price
AP73548706	1,2-Dihexadecanoyl-rac-glycero-3-phospho-rac-(1-glycerol) ammonium salt	73548-70-6	Price
IAR4247444	1-Palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-(1-glycerol) ammonium salt		Price

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