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Photoinduced Nitric Oxide Release From a Nitrobenzene Derivative in Mitochondria

Taeko Horinouchi, Hidehiko Nakagawa, Takayoshi Suzuki, Kiyoshi Fukuhara, Naoki Miyata

Chemistry. 2011 Apr 18;17(17):4809-13.

PMID: 21404338

Abstract:

We report a novel NO donor (RpNO), containing a 2,6-dimethylnitrobenzene moiety for photocontrollable NO release and a rhodamine moiety for targeting to mitochondria. Photorelease of NO from RpNO in aqueous solution was confirmed by means of ESR analysis. Cellular release of NO from RpNO was confirmed with the aid of DAF-FM DA, an NO-specific fluorescence probe. RpNO was colocalized with MitoTracker Green FM, a mitochondrial stain, in HCT116 colon cancer cells and exhibited photodependent cytotoxicity. Our results indicate that RpNO is an effective NO donor for time-controlled, mitochondria-specific NO treatment.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP254109223-C DAF-FM DA solution DAF-FM DA solution 254109-22-3 Price
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