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Piceatannol Protects Human Retinal Pigment Epithelial Cells Against Hydrogen Peroxide Induced Oxidative Stress and Apoptosis Through Modulating PI3K/Akt Signaling Pathway

Yiming Hao, Jie Liu, Ziyuan Wang, Liangli Lucy Yu, Jing Wang

Nutrients. 2019 Jul 4;11(7):1515.

PMID: 31277394

Abstract:

This study investigated the protective effect and the molecular mechanism of piceatannol on hydrogen peroxide (H2O2)-induced retinal pigment epithelium cell (ARPE-19) damage. Piceatannol treatment significantly inhibited H2O2-induced RPE cell death and reactive oxygen species (ROS) generation by 64.4% and 75.0%, respectively. Results of flow cytometry showed that H2O2-induced ARPE-19 cells apoptosis was ameliorated by piceatannol supplementation, along with decreased relative protein expressions of Bax/Bcl-2, Cleave-Caspase-3, and Cleave-PARP. Moreover, piceatannol treatment induced NF-E2-related factor 2 (Nrf2) signaling activation, which was evidenced by increased transcription of anti-oxidant genes, glutamate-cysteine ligase catalytic subunit (GCLc), SOD, and HO-1. Knockdown of Nrf2 through targeted siRNA alleviated piceatannol-mediated HO-1 transcription, and significantly abolished piceatannol-mediated cytoprotection. LY294002 (PI3K inhibitor) dramatically blocked piceatannol-mediated increasing of Nrf2 nuclear translocation, HO-1 expression, and cytoprotective activity, indicating the involvement of PI3K/Akt pathway in the cytoprotective effect of piceatannol. The results from this suggest the potential of piceatannol in reducing the risk of age-related macular degeneration.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP10083246-B Piceatannol Piceatannol 10083-24-6 Price
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