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Poly(colloid)s: "Polymerization" of Poly(l-tyrosine)-silica Composite Particles Through the Photoinduced Cross-Linking of Unmodified Proteins Method

Poly(colloid)s: "Polymerization" of Poly(l-tyrosine)-silica Composite Particles Through the Photoinduced Cross-Linking of Unmodified Proteins Method

Cornelia Rosu, Rafael Cueto, Paul S Russo

Langmuir. 2016 Aug 23;32(33):8392-402.

PMID: 27504929

Abstract:

Photoinduced cross-linking of unmodified proteins, PICUP, was extended to core-shell silica-polypeptide composite particles to produce poly(colloid)s. Silica particles coated with poly(l-tyrosine), PTYR-SiO2, served as the monomer units. The PICUP reaction accomplished the formation of dityrosil linkages between the tyrosine units by illumination of photo-oxidizing ruthenium(II) bipyridyl catalyst under physiological conditions. The PICUP method was compared with an enzymatic route intermediated by horseradish peroxidase as catalyst. The PTYR-SiO2 particles feature high PTYR content in the shell, which facilitated the formation of heavily cross-linked but unstructured aggregates. After magnetic alignment of superparamagnetic PTYR-SiO2-cobalt composite particles, only the PICUP approach enabled the preparation of isolated chain-like poly(colloid)s. The cross-linking products were confirmed by FTIR. The native secondary structure of poly(l-tyrosine) is preserved in these poly(colloid)s. Because the PICUP reaction does not require the modification of the polypeptide structure, the cross-linked PTYR will retain its characteristic functions as a poly(amino acid). The PICUP method opens the door to a variety of PTYR-based poly(colloid) architectures.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP25619787	Poly-L-tyrosine		25619-78-7	Price

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