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Polyamine/salt-assembled Microspheres Coated With Hyaluronic Acid for Targeting and pH Sensing

Pan Zhang, Hui Yang, Guojun Wang, Weijun Tong, Changyou Gao

Colloids Surf B Biointerfaces. 2016 Jun 1;142:223-229.

PMID: 26954089

Abstract:

The poly(allylamine hydrochloride)/trisodium citrate aggregates were fabricated and further covalently crosslinked via the coupling reaction of carboxylic sites on trisodium citrate with the amine groups on polyamine, onto which poly-L-lysine and hyaluronic acid were sequentially assembled, forming stable microspheres. The pH sensitive dye and pH insensitive dye were further labeled to enable the microspheres with pH sensing property. Moreover, these microspheres could be specifically targeted to HeLa tumor cells, since hyaluronic acid can specifically recognize and bind to CD44, a receptor overexpressed on many tumor cells. Quantitative pH measurement by confocal laser scanning microscopy demonstrated that the microspheres were internalized into HeLa cells, and accumulated in acidic compartments. By contrast, only a few microspheres were adhered on the NIH 3T3 cells surface. The microspheres with combined pH sensing property and targeting ability can enhance the insight understanding of the targeted drug vehicles trafficking after cellular internalization.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
LS775181 Poly(fluorescein isothiocyanate allylamine hydrochloride) Poly(fluorescein isothiocyanate allylamine hydrochloride) Price
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