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Polyvinylpyrrolidone-coated Silica Packings for Chromatography of Proteins and Peptides

A Kurganov, Y u Puchkova, V Davankov, F Eisenbeiss

J Chromatogr A. 1994 Mar 11;663(2):163-74.

PMID: 8173664

Abstract:

Polyvinylpyrrolidone (PVP)-coated silica sorbents were synthesized by interaction of a copolymer of vinyl-methyldiethoxysilane and vinylpyrrolidone with LiChrospher Si 300 and LiChrospher Si 500 silicas. The coating procedure retains the wide-pore structure of the starting silicas, as was shown by nitrogen adsorption, mercury porosimetry and inverse size-exclusion chromatographic measurements. Good selectivity and separation ability of the synthesized packings toward proteins and peptides were demonstrated in the hydrophobic interaction chromatographic mode of separation. Aromatic compounds undergo a specific interaction with bonded PVP chains which can be used for the preconcentration or selective recovery of polycyclic aromatic hydrocarbons.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP2031621 Methyldiethoxysilane Methyldiethoxysilane 2031-62-1 Price
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