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Porcine Brain Natriuretic Peptide-Like Immunoreactivity in Rat Tissues

M Sone, K Totsune, K Takahashi, M Ohneda, K Itoi, O Murakami, K Yoshinaga, T Mouri

Peptides. Nov-Dec 1991;12(6):1333-5.

PMID: 1815220

Abstract:

The presence of immunoreactive porcine brain natriuretic peptide in rat tissues was studied with a specific radioimmunoassay for porcine brain natriuretic peptide-26. The cross-reactivity of the antiserum used was less than 0.001% with rat atrial natriuretic peptide, rat brain natriuretic peptide-32 and rat brain natriuretic peptide-45. Immunoreactive porcine brain natriuretic peptide was detectable in various tissues of the rat, and high concentrations of immunoreactive porcine brain natriuretic peptide were found in the brain and cardiac atrium, with the highest level in the hypothalamus (159 +/- 30 fmol/gram wet tissue, mean +/- SEM, n = 4). Reverse phase high performance liquid chromatography showed that the immunoreactive porcine brain natriuretic peptide of the whole brain and heart extracts eluted mainly at an identical position to synthetic porcine brain natriuretic peptide-26. These findings indicate that porcine brain natriuretic peptide-like substance, distinct from rat brain natriuretic peptide, is present in high concentrations in the rat brain and cardiac atrium.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP88898173 Atrial Natriuretic Peptide rat Atrial Natriuretic Peptide rat 88898-17-3 Price
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