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Preliminary Biological Evaluation of Poli(amidoamine) (PAMAM) Dendrimer G3.5 on Selected Parameters of Rat Liver Mitochondria

Preliminary Biological Evaluation of Poli(amidoamine) (PAMAM) Dendrimer G3.5 on Selected Parameters of Rat Liver Mitochondria

Magdalena Labieniec, Teresa Gabryelak

Mitochondrion. 2008 Sep;8(4):305-12.

PMID: 18662806

Abstract:

Polyamidoamino (PAMAM) dendrimer of generation 3.5 (G3.5) specific interactions with rat liver mitochondria were studied. Selected parameters of mitochondria (transmembrane potential and uptake of Ca2+ ions) were investigated after the exposure to G3.5 used at the concentration of 10, 30 and 50 microM and Ca2+ ions used at 1mM. The times of preincubation of isolated liver mitochondria with dendrimer were 5, 15 and 30 min at room temperature. The mitochondrial membrane potential was monitored spectrofluorimetrically by fluorescence quenching of Rhodamine 123 (Rh 123). The changes in calcium homeostasis upon dendrimer exposure were detected using flow cytometric analysis with Fluo-3. On the one hand the obtained results revealed an impact of the tested chemical on rat liver mitochondrial function. We found that dendrimer G3.5 in a concentration above 10 microM contributes to the reduction in the transmembrane potential and hinders in the influx of Ca2+ ions to mitochondria added externally, or accelerates their efflux from mitochondria. The most effective preincubation time was observed to be 15 min for all tested concentrations. On the other hand the combined treatment of dendrimer G3.5 with Ca2+ demonstrated the protective effect of G3.5 against mitochondrial depolarization caused by calcium ions. These preliminary studies suggest that tested dendrimer can significantly affect the mitochondria and modulate their functionality.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP313691804	Thiophosphoryl-PMMH-24 dendrimer, generation 3.5		313691-80-4	Price

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