Home
Resources
Publications
Preparation and Characterization of a Glucose-Responsive Insulin-Releasing Polymer Device

Preparation and Characterization of a Glucose-Responsive Insulin-Releasing Polymer Device

D Shiino, Y Murata, K Kataoka, Y Koyama, M Yokoyama, T Okano, Y Sakurai

Biomaterials. 1994 Jan;15(2):121-8.

PMID: 8011858

Abstract:

A new glucose-responsive insulin delivery system composed of phenylboronic acid (PBA) groups was prepared and investigated. Complexation of various diol-containing molecules with PBA gel beads was evaluated using frontal chromatography. The structural features of the diol-containing molecules strongly influenced their binding to PBA gel beads. In particular, open-chain monosaccharides demonstrated higher association constants (ca 9.5 x 10(2) to 5.1 x 10(3) l/mol) than glucose (ca 6.3 x 10(2) l/mol). Furthermore, a model system utilizing a fluorescent derivative of tris(hydroxymethyl)aminomethane was synthesized and bound to PBA gel beads. The molecules were released in a pulsatile manner in response to glucose. In addition, gluconic acids were chemically attached to insulin molecules. The modified insulin, containing two gluconic acid units per insulin molecule, was isolated using ion-exchange chromatography. This gluconic acid-modified insulin (G-Ins) was bound onto a PBA gel column, and the G-Ins release profile in response to varying glucose concentrations was investigated. The results demonstrate that the PBA gel beads release G-Ins in response to glucose concentration. Thus, this new system may be applied for self-regulated insulin delivery.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP87199153	3-(Hydroxymethyl)phenylboronic acid		87199-15-3	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: