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[Preparation and Identification of Jujuboside A Artificial Antigen]

[Preparation and Identification of Jujuboside A Artificial Antigen]

Degeniee, Wei-Ku Zhang, Hao-Yi Qiao, Hui-Hua Qu, Yan Zhao, Qing-Guo Wang, Jie-Kun Xu

Zhongguo Zhong Yao Za Zhi. 2016 May;41(10):1880-1883.

PMID: 28895337

Abstract:

Immunogenic antigen (jujuboside A-BSA) and coating antigen (jujuboside A-OVA) of jujuboside A were synthesized by sodium periodate oxidation method for the first time. Jujuboside A artificial antigen was confirmed by matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF-MS). The titer and specificity of the antibody in serum of immunized mice were detected by enzyme-linked immunosorbent assay (ELISA). The corrected relation curve of inhibition rate showed that the antibody against Jujuboside A obtained from immunized mice could bind to jujuboside A and the titer was up to 1∶4 000. The jujuboside A artificial antigen was synthesized, which can be used further to preparation of monoclonal antibody and the pharmacokinetics study of jujuboside A in laboratory animals.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP55466041	Jujuboside A		55466-04-1	Price

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