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Preparation and Identification of Monoclonal Antibodies Against Human Brain-Derived Neurotrophic Factor

Preparation and Identification of Monoclonal Antibodies Against Human Brain-Derived Neurotrophic Factor

Jin Su, Ya-Li Zhang, Chang-Xuan You, Jin-Ya Wu, Xiao-Yan Che, Xiao-Ning Wang

Di Yi Jun Yi Da Xue Xue Bao. 2002 Oct;22(10):944-5.

PMID: 12377629

Abstract:

Objective:
To prepare monoclonal antibodies (mAbs) against human brain-derived neurotrophic factor (BDNF).
Methods:
BALB/C mice were immunized with purified BDNF protein in conjunction with acid-treated Salmonella (antigen:thallus=1:5), and mAbs were subsequently derived by hybridoma technique.
Results:
Three hybridoma cell lines secreting anti-BDNF mAbs were obtained, designated as B1, B2 and 4D1 respectively and all categorized into IgG1 subtype. The titers of the mAbs in the ascitic fluid of the rats ranged from 1x10(6) to 1x10(5), and their relative affinities were B2>B1>4D1.
Conclusion:
mAbs against BDNF are successfully prepared, which can be instrumental for further study of the expression and distribution of BDNF in vivo, and the detection of BDNF produced by genetic engineering.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248136	Monoclonal Anti-BDNF antibody produced in mouse			Price

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