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Preparation and Mounting of Adult Drosophila Structures in Canada Balsam

Preparation and Mounting of Adult Drosophila Structures in Canada Balsam

David L Stern, Elio Sucena

Cold Spring Harb Protoc. 2012 Mar 1;2012(3):373-5.

PMID: 22383636

Abstract:

The Drosophila cuticle carries a rich array of morphological details. Thus, cuticle examination has had a central role in the history of genetics. To prepare fine "museum-quality," permanent slides, it is best to mount specimens in Canada Balsam. It is difficult to give precise recipes for Canada Balsam, because every user seems to prefer a slightly different viscosity. Dilute solutions spread easily and do not dry too rapidly while mounting specimens. The disadvantage is that there is actually less Balsam in a "drop" of the solution, and when dried, it can contract from the sides of the coverslip, sometimes disturbing the specimen. Unfortunately, there is no substitute for experience when using Canada Balsam. This protocol describes a procedure for mounting adult cuticles in Canada Balsam.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP8007474-B	Canada balsam solution		8007-47-4	Price

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