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Preparation of 3-ketovalidoxylamine A C-N Lyase Substrate: N-p-nitrophenyl-3-ketovalidamine by Stenotrophomonas Maltrophilia CCTCC M 204024

Preparation of 3-ketovalidoxylamine A C-N Lyase Substrate: N-p-nitrophenyl-3-ketovalidamine by Stenotrophomonas Maltrophilia CCTCC M 204024

Jian-Fen Zhang, Yu-Guo Zheng, Zhi-Qiang Liu, Yin-Chu Shen

Appl Microbiol Biotechnol. 2007 Jan;73(6):1275-81.

PMID: 17058074

Abstract:

3-Ketovalidoxylamine A C-N lyase is one of three key enzymes in the production of valienamine, which is a potent glucosidase inhibitor from validamycin A. N-p-Nitrophenyl-3-ketovalidamine, used as the substrate of 3-ketovalidoxylamine A C-N lyase, was prepared from N-p-nitrophenylvalidamine with free cells of Stenotrophomonas maltrophilia CCTCC M 204024. The yield and selectivity of N-p-nitrophenyl-3-ketovalidamine from cells were improved by treatment with 10 mM ethylenediaminetetraacetic acid. The optimal pH and temperature for N-p-nitrophenyl-3-ketovalidamine formation was pH 6.0 and 30 degrees C, respectively. N-p-Nitrophenyl-3-ketovalidamine was formed with a yield of 0.68 in the first batch.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP59837148	p-Nitrophenyl galacto-N-bioside		59837-14-8	Price

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