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Preparation of Ethyl Magnesium Bromide for Regiospecific Analysis of Triacylglycerols

Preparation of Ethyl Magnesium Bromide for Regiospecific Analysis of Triacylglycerols

Yasuhiro Ando, Yuki Tomita, Yusuke Haba

J Oleo Sci. 2008;57(8):459-62.

PMID: 18622130

Abstract:

This paper presents a procedure for preparation of a Grignard reagent, ethyl magnesium bromide, used for partial deacylation of triacylglycerols (TAG) in their regiospecific analysis. Magnesium turnings were reacted with ethereal solution of bromoethane in a screw-capped test tube to synthesize 2 mL of 1 M ethyl magnesium bromide. Continuously stirred with a vortex mixer, the reaction smoothly proceeded at room temperature. Regiospecific analysis of 1,3-distearoyl-2-oleoylglycerol using this product showed that fatty acid compositions of the sn-1(3) and sn-2 positions were contaminated by less than 2 mol% of fatty acids migrated from isomeric positions. The analyses of lard and cod liver/mackerel oil TAG showed typical distribution patterns of 16:0, 22:5n-3 and 22:6n-3 in pig and fish depot TAG. These results confirmed the view that the freshly prepared reagent is usable for regiospecific analysis of TAG.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP480438446	(1,3-Dioxan-2-ylethyl)magnesium bromide solution		480438-44-6	Price
AP7789482	Magnesium bromide		7789-48-2	Price

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