Home
Resources
Publications
Preparation of Nafion/Polycation Layer-by-Layer Films for Adsorption and Release of Insulin

Preparation of Nafion/Polycation Layer-by-Layer Films for Adsorption and Release of Insulin

Kentaro Yoshida, Katsuhiko Sato, Tetsuya Ono, Takenori Dairaku, Yoshitomo Kashiwagi

Polymers (Basel). 2018 Jul 24;10(8):812.

PMID: 30960737

Abstract:

Thin films were prepared using layer-by-layer (LbL) deposition of Nafion (NAF) and polycations such as poly(allylamine hydrochloride) (PAH), poly(ethyleneimine) (PEI), and poly(diallydimethylammonium chloride) (PDDA). Insulin was then adsorbed on the NAF-polycation LbL films by immersion in an insulin solution. The NAF-polycation LbL films were characterized using a quartz crystal microbalance and an atomic force microscope. The release of insulin from the LbL films was characterized using UV-visible adsorption spectroscopy and fluorescence emission spectroscopy. The greatest amount of insulin was adsorbed on the NAF-PAH LbL film. The amount of insulin adsorbed on the (NAF/PAH)₅NAF LbL films by immersion in a 1 mg mL-1 insulin solution at pH 7.4 was 61.8 µg cm-2. The amount of insulin released from the LbL films was higher when immersed in insulin solutions at pH 2.0 and pH 9.0 than at pH 7.4. Therefore, NAF-polycations could be employed as insulin delivery LbL films under mild conditions and as an insulin release control system according to pH change.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP30551894	Poly(allylamine) solution		30551-89-4	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: