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Preparation of Solid-Phase Immunosorbents by Coupling Human Serum Proteins to Cyanogen Bromide-Activated Agarose

J D Sipe, F V Schaefer

Appl Microbiol. 1973 Jun;25(6):880-4.

PMID: 4197765

Abstract:

The method of preparing solid-phase immunosorbents by covalently attaching proteins from whole human serum to cyanogen bromide-activated agarose has been investigated to determine optimum concentrations of cyanogen bromide and protein, and the optimum pH for the maximum attachment of proteins from serum. Two systems in which the above immunosorbents have proved useful are described: the removal of antibodies to normal serum proteins from anti-hepatitis B serum and the removal of light chain antibodies from anti-human immunoglobulin M serum.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP68987326-A Cyanogen bromide-activated Agarose Cyanogen bromide-activated Agarose 68987-32-6 Price
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