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Preparation of Tyr-C-peptide From Genetically Altered Human Insulin Precursor

Preparation of Tyr-C-peptide From Genetically Altered Human Insulin Precursor

H Sun, J Tang, M Hu

Appl Biochem Biotechnol. 1995 Dec;55(3):167-74.

PMID: 8579343

Abstract:

C-peptide radioimmunoassay (C-peptide RIA) is widely used in determination of pancreatic B-cell secretion activity. 125I labeled Tyr-C-peptide is indispensable in C-peptide RIA kit. Herein we discuss a way of obtaining recombinant Tyr-C-peptide. Arg32Tyr human pro-insulin mutant (R32Y-proinsulin) gene was constructed by site-directed mutagenesis and overexpressed in Escherichia coli. Purified R32Y-proinsulin was converted to insulin and Tyr-C-peptide by trypsin and carboxypeptidase B codigestion. Tyr-C-peptide was isolated through reverse-phase HPLC (RP-HPLC) and identified by C-peptide RIA and amino acid analysis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP139532119	Tyr-C-Peptide human		139532-11-9	Price

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