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Preparation, Optimization and Cellular Uptake Study of Tanshinone I Nanoemulsion Modified With Lactoferrin for Brain Drug Delivery

Preparation, Optimization and Cellular Uptake Study of Tanshinone I Nanoemulsion Modified With Lactoferrin for Brain Drug Delivery

Yumei Wu, Bing Zhang, Dereje Kebebe, Lili Guo, Hong Guo, Nan Li, Jiaxin Pi, Dongli Qi, Pan Guo, Zhidong Liu

Pharm Dev Technol. 2019 Oct;24(8):982-991.

PMID: 31107131

Abstract:

Tanshinone I (TSI) is one of the bioactive compound obtained from the root of Salvia miltiorrhiza which is a well-known traditional Chinese medicine (TCM) used for the treatment of various diseases. Although TSI possesses several pharmacological effects, it has poor water solubility, blood-brain barrier (BBB) permeability and brain bioavailability. Therefore, in the present study, we developed TSI nanoemulsion (TSI-NE) modified with a brain targeting ligand (Lactoferrin (Lf)) to improve the BBB permeability. Pseudo-ternary phase diagrams were used to optimize the formulation. The optimal TSI-NE and TSI-Lf-NE were prepared and characterized. Finally, the uptake of TSI-Lf-NE by mouse brain microvascular endothelial cell line (bEnd.3 cells) was assessed using Coumarin-6 as a fluorescent probe. The results of the study showed that the stable optimal formulation of O/W nanoemulsion was successfully developed and modified with Lf. The cellular uptake study has shown that the fluorescence intensity (FI) increased with time over the incubation period. The FI at all time intervals increased in the following order: Coumarin-6-Solution

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AS26617	Polynuclear aromatic hydrocarbons 6 solution			Price

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