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Preparative Separation of Glycoalkaloids α-solanine and α-chaconine by Centrifugal Partition Chromatography

Preparative Separation of Glycoalkaloids α-solanine and α-chaconine by Centrifugal Partition Chromatography

Jacques Attoumbré, David Lesur, Philippe Giordanengo, Sylvie Baltora-Rosset

J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Nov 1;908:150-4.

PMID: 23040988

Abstract:

The main glycoalkaloids of a commercial potato cultivar, α-chaconine and α-solanine, were extracted from sprouts of Solanum tuberosum cv. Pompadour by a mixture of MeOH/H(2)O/CH(3)COOH (400/100/50, v/v/v). In these conditions, 2.8±0.62g of crude extract were obtained from 50g of fresh sprouts and the total glycoalkaloid content was determined by analytical HPLC at 216.5mg/100g. α-Chaconine and α-solanine were separated in a preparative scale using centrifugal partition chromatography (CPC). In a solvent system composed of a mixture of ethyl acetate/butanol/water (15/35/50, v/v/v), α-chaconine (54mg) and α-solanine (15mg) were successfully isolated from the crude extract in one step of purification. The purity of isolated compounds was determined to be higher than 92% by HPLC analysis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP20562032	α-Chaconine		20562-03-2	Price

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