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Prestaining of Glycoproteins in Sodium Dodecyl Sulfate Polyacrylamide Gels by Dansylhydrazine

Yang Wang, Xuan Zhou, Qing Yu, Yuanmeng Duan, Binbin Huang, Guoying Hong, Ayi Zhou, Litai Jin

Proteomics. 2014 Jun;14(11):1322-7.

PMID: 24668852

Abstract:

A new fluorescent prestaining method for gel-separated glycoproteins in 1D and 2D SDS-PAGE was developed by using dansylhydrazine in this study. The prestained gels could be easily imaged after electrophoresis without any time-consuming steps needed for poststains. As low as 4-8 ng glycoproteins (transferrin, α1-acid glycoprotein) could be selectively detected, which is comparable to that of Pro-Q Emerald 488, one of the most commonly used glycoprotein stain. In addition, a subsequent study of deglycosylation, glycoprotein affinity isolation, and LC-MS/MS analysis was performed to confirm the specificity of the newly developed method.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP33008069 Dansylhydrazine Dansylhydrazine 33008-06-9 Price
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