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Production and Characterization of Rabbit Anti-Idiotypic Antibodies Directed Against a Murine Monoclonal anti-B. Abortus Antibody

R Adone, F Ciuchini, R Bianchi, G Piccininno, C Pistoia

Vet Res Commun. 1995;19(6):457-61.

PMID: 8619283

Abstract:

The protective anti-beta abortus monoclonal antibody ISS/32 (Ab1) was used as an immunogen to induce anti-idiotypic antibodies (Ab2) in rabbits. The purpose was to produce and characterize anti-idiotypic antibodies that share conformational similarity with the corresponding bacterial epitope recognized by Ab1. The rabbit anti-IdAb so induced was isolated and affinity-purified. Its specificity for the paratope of Ab1 was determined by evaluating its ability to compete with B. abortus for binding to Ab1 in a competitive ELISA assay. The anti-idiotypic ISS/32 antibodies were able to compete with B. abortus for binding to Ab1 in a dose-dependent manner. Hence, the data indicated that the rabbit anti-Id ISS/32 antibodies reacted with or near the antigen-binding site of Ab1.

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