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[Production and Identification of Monoclonal Antibody Against N Terminal of AIB1]

[Production and Identification of Monoclonal Antibody Against N Terminal of AIB1]

Qiu-Xia Pang, Xiao-Dong Peng, Ming-Rong Xi, Yue-Ming Zhu, Xu-Hong Peng, Wei Dong, Lin Zhang, Yi Qu

Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Mar;37(2):278-80, 297.

PMID: 16608094

Abstract:

Objective:
To make and identify the monoclonal antibody against AIB1-N.
Methods:
BALB/c mice were immunized with purified GST-AIB1-N protein, McAb against AIB1-N was produced by hybridoma technique. ELISA and Western-blot were used to identify the immunoglobin subtype and specificity. Results A hybridoma cell was successfully produced to secrete the McAb against AIB1-N, which was identified to belong in IgG1 subtype. By western-blot, the McAb against AIB1 displayed strongly specificity and high affinity.
Conclusion:
The McAb against AIB1 protein may be a useful tool for studying the biological properties of AIB1 expression and the clinical laboratory detection.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4246061	Monoclonal Anti-ACTR/AIB1 antibody produced in mouse			Price

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