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Production and Properties of Alpha-Amylase From Bacillus Sp. BKL20

Production and Properties of Alpha-Amylase From Bacillus Sp. BKL20

Olha I Kubrak, Janet M Storey, Kenneth B Storey, Volodymyr I Lushchak

Can J Microbiol. 2010 Apr;56(4):279-88.

PMID: 20453894

Abstract:

As a result of screening Bacillus sp. strains isolated from different natural substrates, strain BKL20 was identified as a producer of a thermostable alkaline alpha-amylase. Maximum production of this alpha-amylase was achieved by optimizing culture conditions. Production of alpha-amylase seemed to be independent of the presence of starch in the culture medium and was stimulated by the presence of peptone (0.3%, m/v) and yeast extract (0.2%, m/v). The enzyme was thermostable and retained amylolytic activity after 30 min of incubation at 60 and 70 degrees C. High activity was maintained over a broad pH range, from 6.0 to 11.0, and the enzyme remained active after alkaline incubation for 24 h. Bacillus sp. BKL20 alpha-amylase was not stimulated by Ca2+ or other bivalent metal cations and was not inhibited by EGTA or EDTA at 1-10 mmol/L, suggesting that this alpha-amylase is a Ca2+-independent enzyme. It also showed good resistance to both oxidizing (H2O2) and denaturating (urea) agents.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9000855-D	α-Amylase from Bacillus sp.		9000-85-5	Price

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