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Production of 13S-hydroxy-9(Z)-octadecenoic Acid From Linoleic Acid by Whole Recombinant Cells Expressing Linoleate 13-hydratase From Lactobacillus Acidophilus

Production of 13S-hydroxy-9(Z)-octadecenoic Acid From Linoleic Acid by Whole Recombinant Cells Expressing Linoleate 13-hydratase From Lactobacillus Acidophilus

Ji-Young Park, Seon-Hwa Lee, Kyoung-Rok Kim, Jin-Byung Park, Deok-Kun Oh

J Biotechnol. 2015 Aug 20;208:1-10.

PMID: 26015260

Abstract:

Linoleate 13-hydratase from Lactobacillus acidophilus LMG 11470 converted linoleic acid to hydroxyl fatty acid, which was identified as 13S-hydroxy-9(Z)-octadecenoic acid (13-HOD) by GC-MS and NMR. The expression of linoleate 13-hydratase gene in Escherichia coli was maximized by using pACYC plasmid and super optimal broth with catabolite repression (SOC) medium containing 40mM Mg(2+). To optimize induction conditions, recombinant cells were cultivated at 37°C, 1mM isopropyl-β-d-thiogalactopyranoside was added at 2h, and the culture was further incubated at 16°C for 18h. Recombinant cells expressing linoleate 13-hydratase from L. acidophilus were obtained under the optimized expression conditions and used for 13-HOD production from linoleic acid. The optimal reaction conditions were pH 6.0, 40°C, 0.25% (v/v) Tween 40, 25gl(-1) cells, and 100gl(-1) linoleic acid, and under these conditions, whole recombinant cells produced 79gl(-1) 13-HOD for 3h with a conversion yield of 79% (w/w), a volumetric productivity of 26.3gl(-1)h(-1), and a specific productivity of 1.05g g-cells(-1)h(-1). To the best of our knowledge, the recombinant cells produced hydroxy fatty acid with the highest concentration and productivity reported so far.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411427	SOC Medium			Price

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