Home
Resources
Publications
Production of Anti-Glucagon Sera With a C-terminal Fragment of Pancreatic Glucagon

Production of Anti-Glucagon Sera With a C-terminal Fragment of Pancreatic Glucagon

K Imagawa, T Nishino, S Shin, S Uehata, E Hashimura, C Yanaihara, N Yanaihara

Endocrinol Jpn. 1979 Feb;26(1):123-31.

PMID: 86440

Abstract:

The C-terminal region-sepcific anti-glucagon sera were raised in rabbits using as immunogen, and conjugate of BSA and a C-terminal fragment of pancreatic glucagon. The hapten was prepared by trypsin digestion of the glucagon, which was proved to be a 1:3 mixture of glucagon (18--29) and (19--29). Six rabbits were immunized by subcutaneous injection of an emulsion of the conjugate with complete Freund's adjuvant and five of the rabbits produced antibodies to the glucagon (GC-1, GC-2, GC-3, GC-5 and GC-6). For comparison, rabbit antisera were also produced against glucagon polymer (GA-10) and syrupy glucagon fibrils (PGA-2). All these antisera as well as the pancreatic glucagon-specific antiserum 30 K were characterized with dog gut-extract (gut-GLI) and glucagon-related peptide fragments in the radioimmunoassay systems. The assay systems utilized 125 I-monosubstituted pancreatic glucagon as tracer and human mono-component glucagon as standard. All sera of the GC-series crossreacted with the dog gut-extract very weakly and antisera GC-5 and GC-6 exhibited the lowest crossreactivities with the extract, which were shown to be as low as that of 30k. Characterization of the antiserum GC-5 with purified glucagon related fragments indicated that the major antigenic determinant located exactly in the C-terminal region of glucagon. The present results clearly showed high efficiency of the use of the glucagon C-terminal fragment as hepatenic immunogen in obtaining the C-terminal region-specific, i.e., pancreatic glucagon-specific antisera.

Chemicals Related in the Paper:

Catalog Number	Product Name	Price
IAR42412387	Anti-Acinus, C-Terminal antibody produced in rabbit	Price
IAR42412487	Anti-FLIPα, C-Terminal antibody produced in rabbit	Price
IAR42413902	Anti-Palladin (C-terminal) antibody produced in rabbit	Price
IAR4247037	Anti-VAC14 (C-terminal) antibody produced in rabbit	Price
IAR4248036	Anti-ZNF746 (C-terminal region) antibody produced in rabbit	Price
IAR4249151	Anti-MIB2 (C-terminal) antibody produced in rabbit	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: