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Production of Antibody to Lipopolysaccharide (LPS) After Immunization With a LPS-polymyxin B-agarose Immunogen

Production of Antibody to Lipopolysaccharide (LPS) After Immunization With a LPS-polymyxin B-agarose Immunogen

L K Ryan, M H Karol

J Appl Bacteriol. 1988 Jun;64(6):487-95.

PMID: 3049507

Abstract:

A method was devised to produce antibodies to lipopolysaccharide (LPS) in guinea-pigs following a single immunization. The antigen was prepared by mixing polymyxin B-agarose with LPS from Escherichia coli O55:B5. Use of the agarose support allowed purification of the complex by simple washing procedures. Twenty-nine days after a single injection of the immunogen mixed with Freund complete adjuvant all animals demonstrated antibody to the LPS portion of the complex. No antibodies were detected to the polymyxin B component. Typical titres of LPS as measured by ELISA were 2(11). After, a booster immunization, titres of LPS antibody were further increased and a greater avidity was noted. In contrast to other methods which have been employed for production of antibody to LPS, use of the polymyxin B-agarose complex has the following advantages: ease of antigen preparation, ready purification of the complex, potent immunostimulation, and under the conditions employed here, LPS-specific antibody production, without accompanying antibody to polymyxin B.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411071	Polymyxin B-Agarose			Price

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