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Production of Biodiesel From Plant Oil Hydrolysates Using an Aspergillus Oryzae Whole-Cell Biocatalyst Highly Expressing Candida Antarctica Lipase B

Production of Biodiesel From Plant Oil Hydrolysates Using an Aspergillus Oryzae Whole-Cell Biocatalyst Highly Expressing Candida Antarctica Lipase B

Daisuke Adachi, Shinji Hama, Kazunori Nakashima, Takayuki Bogaki, Chiaki Ogino, Akihiko Kondo

Bioresour Technol. 2013 May;135:410-6.

PMID: 22850174

Abstract:

For enzymatic biodiesel production from plant oil hydrolysates, an Aspergillus oryzae whole-cell biocatalyst that expresses Candida antarctica lipase B (r-CALB) with high esterification activity was developed. Each of soybean and palm oils was hydrolyzed using Candida rugosa lipase, and the resultant hydrolysates were subjected to esterification where immobilized r-CALB was used as a catalyst. In esterification, r-CALB afforded a methyl ester content of more than 90% after 6 h with the addition of 1.5 M equivalents of methanol. Favorably, stepwise additions of methanol and a little water were unnecessary for maintaining the lipase stability of r-CALB during esterification. During long-term esterification in a rotator, r-CALB can be recycled for 20 cycles without a significant loss of lipase activity, resulting in a methyl ester content of more than 90% even after the 20th batch. Therefore, the presented reaction system using r-CALB shows promise for biodiesel production from plant oil hydrolysates.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9001621-E	Lipase B Candida antarctica, recombinant from Aspergillus oryzae		9001-62-1	Price

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