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Production of γ-cyclodextrin by Bacillus Cereus Cyclodextrin Glycosyltransferase Using Extractive Bioconversion in Polymer-Salt Aqueous Two-Phase System

Production of γ-cyclodextrin by Bacillus Cereus Cyclodextrin Glycosyltransferase Using Extractive Bioconversion in Polymer-Salt Aqueous Two-Phase System

Yu Kiat Lin, Pau Loke Show, Yee Jiun Yap, Arbakariya B Ariff, Mohammad Suffian Mohammad Annuar, Oi Ming Lai, Teck Kim Tang, Joon Ching Juan, Tau Chuan Ling

J Biosci Bioeng. 2016 Jun;121(6):692-696.

PMID: 26702953

Abstract:

Aqueous two-phase system (ATPS) extractive bioconversion provides a technique which integrates bioconversion and purification into a single step process. Extractive bioconversion of gamma-cyclodextrin (γ-CD) from soluble starch with cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) enzyme derived from Bacillus cereus was evaluated using polyethylene glycol (PEG)/potassium phosphate based on ATPS. The optimum condition was attained in the ATPS constituted of 30.0% (w/w) PEG 3000 g/mol and 7.0% (w/w) potassium phosphate. A γ-CD concentration of 1.60 mg/mL with a 19% concentration ratio was recovered after 1 h bioconversion process. The γ-CD was mainly partitioned to the top phase (YT=81.88%), with CGTase partitioning in the salt-rich bottom phase (KCGTase=0.51). Repetitive batch processes of extractive bioconversion were successfully recycled three times, indicating that this is an environmental friendly and a cost saving technique for γ-CD production and purification.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS741353	γ-Cyclodextrin polymer, soluble			Price

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