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Production of D-Glucuronic Acid From Myo-Inositol Using Escherichia Coli Whole-Cell Biocatalyst Overexpressing a Novel Myo-Inositol Oxygenase From Thermothelomyces Thermophile

Production of D-Glucuronic Acid From Myo-Inositol Using Escherichia Coli Whole-Cell Biocatalyst Overexpressing a Novel Myo-Inositol Oxygenase From Thermothelomyces Thermophile

Fei Teng, Ran You, Meirong Hu, Weifeng Liu, Lei Wang, Yong Tao

Enzyme Microb Technol. 2019 Aug;127:70-74.

PMID: 31088620

Abstract:

D-glucuronic acid (GlcUA) is an important intermediate with numerous applications in the food, cosmetics, and pharmaceutical industries. Its biological production routes which employ myo-inositol oxygenase (MIOX) as the key enzyme are attractive. In this study, five diverse MIOX-encoding genes, from Cryptococcus neoformans, Chaetomium thermophilum, Arabidopsis thaliana, Thermothelomyces thermophila, and Mus musculus were overexpressed in Escherichia coli, respectively. A novel MIOX from Thermothelomyces thermophila (TtMIOX) exhibited high specific activity, and efficiently converted myo-inositol to GlcUA. Meanwhile, the degradation of GlcUA was inhibited by inactivation of uxaC from the Escherichia coli genome. Finally, the BWΔuxaC whole-cell biocatalyst harboring TtMIOX resulted in the production of 106 g/L GlcUA within 12 h in a 1-L bioreactor, corresponding to a conversion of 91% and productivity of 8.83 g/L/h. This study provides a feasible method for the industrial production of GlcUA.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP6556123	D-Glucuronic acid		6556-12-3	Price

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