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Production of Fumonisin B1 by Fusarium Moniliforme NRRL 13616 in Submerged Culture

Production of Fumonisin B1 by Fusarium Moniliforme NRRL 13616 in Submerged Culture

M A Jackson, G A Bennett

Appl Environ Microbiol. 1990 Aug;56(8):2296-8.

PMID: 2403248

Abstract:

Fumonisin B1, a recently discovered mycotoxin, was synthesized by submerged cultures of Fusarium moniliforme NRRL 13616 grown for 29 days at 28 degrees C and 220 rpm in a basal salts medium (pH 5.0) supplemented with 90 g of glucose per liter and 3.5 g of ammonium sulfate per liter. Under these culture conditions, 74 +/- 23 micrograms of fumonisin B1 per ml was produced by 29-day-old F. moniliforme NRRL 13616 cultures. Fumonisin B1 was detected in liquid culture extracts by high-performance thin-layer chromatography. Fumonisin B1 was confirmed and quantitated by gas chromatography and gas chromatography-mass spectral analysis of the trimethylsilyl derivative. The use of a defined medium for producing fumonisin B1 in a submerged culture facilitates its isolation and provides an excellent method for conducting biosynthetic studies.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP116355830-A	Fumonisin B1 from Fusarium moniliforme		116355-83-0	Price

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