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Production of Monoclonal Anti-Thymine-Dimer Antibody and Its Use for Detection of STR Alleles

Production of Monoclonal Anti-Thymine-Dimer Antibody and Its Use for Detection of STR Alleles

M Fukuda, Y Tamaki, T Kishida, W Wang

Nihon Hoigaku Zasshi. 1996 Oct;50(5):343-8.

PMID: 8952334

Abstract:

To simplify the manual detection of STRs, we developed an immunoblotting method using an anti-thymine-dimer antibody. Upon ultraviolet irradiation, adjacent thymine residues in a DNA strand readily form thymine dimers which are detectable by anti-thymine-dimer antibody. We simply irradiated the supernatant of a boiled suspension of P3U1 myeloma cells with ultraviolet rays, and immunized BALB/c mice with it. By fusing their popliteal lymph node cells with the P3U1 myeloma cell line, we produced a monoclonal anti-thymine-dimer antibody. Using this antibody, we were able to detect STR alleles containing AAAG, AAGG, or AATG repeats by immunoblotting. Our method is well suited for use in parentage testing.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42412955	Anti-Thymine Dimer antibody, Mouse monoclonal			Price

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