Home
Resources
Publications
Production of Pig Liver Esterase in Batch Fermentation of E. Coli Origami

Production of Pig Liver Esterase in Batch Fermentation of E. Coli Origami

Elke Brüsehaber, Anja Schwiebs, Marlen Schmidt, Dominique Böttcher, Uwe T Bornscheuer

Appl Microbiol Biotechnol. 2010 May;86(5):1337-44.

PMID: 20024542

Abstract:

The establishment of a fermentation process for the production of pig liver esterase (PLE) in high yields is necessary for industrial applications. In our previous studies, we reported the recombinant expression of PLE in Escherichia coli Origami (DE3) in shake flask. Only a coexpression with chaperones GroEL/ES allowed the production of soluble and active enzyme. The optimization of the cultivation conditions, such as temperature, inducer concentrations, or media compositions to increase enzyme yield in a fermentation process is described here. Using fed-batch fermentation cell densities up to OD = 50 were obtained, but almost no active enzyme was expressed. Only batch fermentation was found suitable for production of active pig liver esterase and cell densities between OD = 7-13 and activities of 300-400 U L(-1) for isoenzyme PLE-1 (gammaPLE) and 1,400 U L(-1) for PLE-5 were obtained after 22 h total cultivation time or 18 h after induction of PLE expression, respectively.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9016186-H	Esterase Isoenzyme 1 porcine liver, recombinant		9016-18-6	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: