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Production of the Apoptotic Cellular Mediator 4-Methylthio-2-oxobutyric Acid by Using an Enzymatic Stirred Tank Reactor With in Situ Product Removal

Production of the Apoptotic Cellular Mediator 4-Methylthio-2-oxobutyric Acid by Using an Enzymatic Stirred Tank Reactor With in Situ Product Removal

Miguel García-García, Irene Martínez-Martínez, Alvaro Sánchez-Ferrer, Francisco García-Carmona

Biotechnol Prog. Jan-Feb 2008;24(1):187-91.

PMID: 18092800

Abstract:

d-Amino acid oxidase (DAAO) was used to study the oxidative deamination of racemic mixtures of d,l-methionine in its soluble and immobilized forms and thus obtain the corresponding alpha-keto acid. The soluble enzyme form was obtained from a Trigonopsis variabilis CBS 4095 extract, free of l-amino acid oxidase, and was co-immobilized with a 200-fold excess of catalase to avoid the undesirable side reaction of H2O2 with the alpha-keto acid, which would otherwise render its corresponding decarboxylated acid, the 3-methylthiopropionic acid (MTPA). With this biocatalyst, quantitative conversion (>98%) of d-methionine into the alpha-keto acid 4-methylthio-2-oxobutyric acid (MTOB) and into MTPA was achieved using 5 mg.mL(-1) of biocatalyst at pH 8.0, 25 degrees C, and pure oxygen at 3 vvm. A stirred tank reactor with in situ product removal (STR-ISPR) was developed to avoid conversion of MTOB into MTPA. The reaction medium was re-circulated through a strong anion exchange column (Amberlite IRA-400). This resulted in the complete removal of MTOB from the reaction medium. After the reaction, the reaction products were eluted sequentially with water (l-methionine), 10 mM HCl (MTPA), and 0.5 M HCl (MTOB). After elution, MTOB was crystallized to its sodium salt.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP600180	2-Oxobutyric acid		600-18-0	Price

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