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Protamine Sulfate Stimulates Degradation of Factor Xa and the Factor Xa-antithrombin Complex

Protamine Sulfate Stimulates Degradation of Factor Xa and the Factor Xa-antithrombin Complex

Martin H Coggin, Rebecca Ahl, Amie Roland, Doris Beck, Arthur S Brecher

Blood Coagul Fibrinolysis. 2011 Jun;22(4):247-53.

PMID: 21297450

Abstract:

The effect of protamine sulfate on factor Xa (FXa) and the factor Xa-antithrombin complex was studied via SDS-PAGE and Western blot analysis. Human factor Xa [(FXaα) ∼52 kDa, FXaβ ∼47 kDa] and human antithrombin (AT ∼55 kDa) form a primary (1°) complex at 109 and 104 kDa, a secondary (2°) complex at 99 and 95 kDa, and a tertiary (3°) complex at 66 and 62 kDa, as detected with polyclonal anti-FXa and anti-AT antibodies. Addition of protamine sulfate stimulates a transformation from free FXaα to FXaβ and degradation thence to inactive FXaγ (∼30 kDa), as well as transformation of FXaα-AT complexes to FXaβ-AT complexes. Additionally, protamine sulfate promotes digestive degradation from 1° to 3° complexes and FXaγ by the active enzyme. It further promotes reduction in total complex formation as a function of stimulation of hydrolysis of FXa moieties from the complex, thereby generating ATM. Protamine sulfate effects are proportional to the protamine sulfate concentration.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9009658	Protamine sulfate		9009-65-8	Price

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