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Protective Effect of calycosin-7-O-β-D-glucopyranoside Against Oxidative Stress of BRL-3A Cells Induced by Thioacetamide

Protective Effect of calycosin-7-O-β-D-glucopyranoside Against Oxidative Stress of BRL-3A Cells Induced by Thioacetamide

Li Jian, Lin Xin, Ma Yufang, Huang Yifan

Pharmacogn Mag. Jul-Sep 2015;11(43):524-32.

PMID: 26246727

Abstract:

Background:
Calycosin-7-O-β-D-glucopyranoside (CG) is a natural isoflavone found in traditional Chinese medicines Astragali Radix (AR).
Objective:
Calycosin-7-O-β-D-glucopyranoside, an isoflavone isolated from AR, has been found to have potent antioxidantive effects. This study was designed to investigate whether CG prevents oxidative stress induced by thioacetamide (TAA).
Materials and methods:
BRL-3A cells were pretreated with different concentrations of CG (10, 20, 40 mg/mL) for 12 h and then exposed to 0.18 mol/L TAA for 2 h. The cell viability were examined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium assay, total antioxidant capacity, malondialdehyde (MDA) and the activity of antioxidant enzymes, including catalase, glutathione peroxidase and superoxide dismutase were determined by microplate method. Reactive oxygen species (ROS) generation was quantified by the 2',7'-dichlorofluorescin-diacetate method. Protein and mRNA expression of CYP2E1 were determined by western blotting and real-time PCR.
Results:
The cell oxidative stress was significantly increased after 2 h of TAA exposure. Pretreatment of BRL-3A cells with CG significantly increased the activities of antioxidant enzymes, scavenged ROS and reduced MDA production. CG decreased the expression of CYP2E1, and ultimately decreased TAA-induced BRL-3A cells oxidative stress.
Conclusions:
Calycosin-7-O-β-D-glucopyranoside has a protective effect against TAA-induced oxidative stress in BRL-3A cells, and that the underlying mechanism involves in scavenging of ROS and the modulating expression of CYP2E1.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP20633674	Calycosin 7-O-β-D-glucopyranoside		20633-67-4	Price

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